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LGTC Order System v.1.3.5 | Updated 2024-07-17 |
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Please log in below. No account? Register your client account here. Dear researcher, Please note, the LGTC will be closed from the 25th of December till the 5th of January. We wish you happy holidays and a wonderful new year. -Sanger sequencing tubes are available outside room R5-20 and Bioanalyzer chips are outside room R5-22. -For internal users, you need to book your time in the Outlook room calendars for the following instruments: Agilent Bioanalyzer, room R-04-021 Qubit, room R-04-021 Light Scanner, room R-04-21 If you don't have access to the room calendar, please contact the LGTC team -For external users, to book your time please contact the LGTC team Contact: info@lumc.nl or 071-5269500/69502 Sanger sequencing Please deliver your Sanger Sequencing samples as usual in R4-21. Tubes and caps are available at R5-20. Recommended Concentration and Volume: Total volume needed is 10ul: 5ul template_concentration + 5ul primer (FINAL primer concentration 1 pmol/ul). Template_concentration is size related: BAC concentration over 500ng/ul Plasmid (over 4kb) concentration 200ng/ul Plasmid (below 4kb) concentration 100-150ng/ul Purified PCR product (>1.5kb): 100ng/ul Purified PCR product (700bp ~ 1.5kb): 50-75ng/ul Purified PCR product (300bp ~ 700bp): 25-50ng/ul Purified PCR product less than 300bp): 10-15ng/ul |
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