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LGTC Order System v.1.3.3 | Updated 2023-01-04 |
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Please log in below. No account? Register your client account here. Dear LGTC user, due to the corona measures we have changed a few procedures listed below. Please read these carefully. -Sanger sequencing tubes and Bioanalyzer chips are available in room R5-20 and can be collected only during office hours. -For internal users, you need to book your time in the Outlook room calendars for the following instruments: Agilent Bioanalyzer, room R-05-002 Qubit, room R-05-002 Light Scanner, room R-04-21 LightCycler, room R-04-21 If you don't have access to the room calendar, please contact the LGTC team -For external users, to book your time please contact the LGTC team Contact: info@lumc.nl or 071-5269500/69502 -Please comply to the COVID guidlines given by the LUMC and the government at all times. Sanger sequencing Please deliver your Sanger Sequencing samples as usual in R4-21. Tubes and caps are available at R5-20. Recommended Concentration and Volume: Total volume needed is 10ul: 5ul template_concentration + 5ul primer (FINAL primer concentration 1 pmol/ul). Template_concentration is size related: BAC concentration over 500ng/ul Plasmid (over 4kb) concentration 200ng/ul Plasmid (below 4kb) concentration 100-150ng/ul Purified PCR product (>1.5kb): 100ng/ul Purified PCR product (700bp ~ 1.5kb): 50-75ng/ul Purified PCR product (300bp ~ 700bp): 25-50ng/ul Purified PCR product less than 300bp): 10-15ng/ul |
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