This week-long course covers the practicalities of single-cell sample preparation and analysis, with a particular focus on single-cell RNA-sequencing (scRNA-seq) libraries. This course is aimed at both wet-lab researchers interested in learning how to analyse their own single-cell data sets, as well as bioinformaticians who are new to single-cell sequencing analysis. Basic knowledge of the programming language R is a prerequisite for participating in the course.